fructosivorans WT and the hndD deletion mutant strains when grown on ethanol as the sole carbon and energy source. In this study, the approach implemented was to compare the two S. Under these conditions, it consumes H 2 to produce reducing equivalents for ethanol production as a fermentative product. Regarding its implication in the bacterial physiology, we previously showed that Hnd, which is non-essential when bacteria grow fermentatively on pyruvate, is involved in ethanol metabolism. The tetrameric cytoplasmic FeFe hydrogenase Hnd from Solidesulfovibrio fructosivorans (formely Desulfovibrio fructosovorans) catalyses H 2 oxidation and couples the exergonic reduction of NAD + to the endergonic reduction of a ferredoxin by using a flavin-based electron-bifurcating mechanism. 3CNRS, Aix-Marseille Univ, Plate-forme Protéomique de l’IMM, FR 3479, Marseille Protéomique (MaP), Marseille, France.2CNRS, Aix-Marseille Univ, Centrale Marseille, ISM2, Marseille, France.

1CNRS, Aix-Marseille Univ, BIP, Marseille, France.Arlette Kpebe 1 †, Chloé Guendon 1 †, Natalie Payne 1,2, Julien Ros 1, Manel Khelil Berbar 3, Régine Lebrun 3, Carole Baffert 1, Laetitia Shintu 2 and Myriam Brugna 1 *

Vector magic 1.15 full